1. Cultures

2. Origin

3. Preparation

4. See also

5. References

Tibicos, or water kefir is a traditional fermented drink made with a symbiotic culture of bacteria and yeasts (SCOBY) held in a polysaccharide biofilm matrix created by the bacteria.

People who do not wish to consume dairy products may find that water kefir provides probiotics without the need for dairy, or tea-cultured products such as kombucha. The finished product, if bottled, will produce a carbonated beverage; it will continue to ferment when bottled, thus producing more carbonation, so bottles need to be capped loosely and allowed to breathe, lest they burst.

Cultures

Tibicos cultures are found around the world, with no two being exactly the same; but typical tibicos have a mix of Lactobacillus, Streptococcus, Pediococcus and Leuconostoc bacteria, with yeasts from Saccharomyces, Candida, Kloeckera and possibly others. Lactobacillus brevis bacteria has been identified as the species responsible for the production of the dextran polysaccharide that forms the "grains".^[1][2]

As with milk kefir "grains", the microbes present in tibicos act in symbiosis to maintain a stable culture. Tibicos can do this in many different sugary liquids, feeding off the sugar to produce lactic acid, alcohol (ethanol), and carbon dioxide gas, which carbonates the drink. They are also known as tibi, water kefir grains, sugar kefir grains, Japanese water crystals and California bees, and in older literature as bébées, African bees, Australian bees, ginger bees, vinegar bees, bees, Japanese beer seeds, beer seeds, beer plant, ale nuts, Jack's Magic Beans, eternity grains,^[3] and balm of Gilead.^[4]

Pidoux (1989) identifies sugary kefir grain with the ginger beer plant.^[2] Certainly, opportunistic bacteria take advantage of this stable symbiotic relation, which might be the reason for the many different names in the scientific literature. Different ingredients or hygienic conditions might also change the fungal and bacteriological composition, leading to the different names.

Origin

At least two references in the scientific literature relate to the origin of the tibi. According to the first paper, tibicos forms on the pads of the Opuntia cactus (from Mexico) as hard granules that can be reconstituted in a sugar-water solution as propagating tibicos. The second paper refers to a specific bacteria cultured from known stocks with properties similar to those in tibi.^[5][6]

Tibicos are used to brew a variety of tepache known as tepache de tibicos.^[7] The ginger beer plant is also a form of tibicos. Kebler attests that they were used in Kentucky circa 1859 to brew a "home drink" and were referred to as "Japanese beer seeds".^[4]

Preparation

The basic preparation method is to add tibicos to a sugary liquid and allow it to ferment 24 to 48 hours. A typical recipe might contain the tibicos culture, a citrus fruit, and water.

It is important to not use ingredients that will inhibit the fermentation, such as chlorine in tap water or preservatives in dried fruit (sulfites). The fruits used may be changed and mixed to create different flavors.

Additional precautions should be taken to keep the cultures healthy. The use of reactive metals such as aluminium, copper, or zinc should be minimized, since the acidity of the solution can draw these metals out, damaging the culture. The beverage should not be stored in reactive metal containers, as these may leach into it over time. Instead, use plastic, lead-free ceramic, or glass containers. Culturing grains in a glass jar and using clean plastic or wooden utensils when handling the grains is recommended.

See also

• Kefir
• Kombucha

References

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• HP1BP3 (gene)
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• Hp1likevirus
• HP 1LK7
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• HP 1LR3
• HP 1LT8
• HP 1LU7
• HP1 (soundtrack)
• HP-20
• HP-20b
• HP 20S