Flight 181 may refer to:

• Lufthansa Flight 181, hijacked on 13 October 1977
• EgyptAir Flight 181, hijacked on 29 March 2016

• Large Looper Moth
• Large loop excision of the transformation zone
• Large Magellenic Cloud
• Large Marge (character)
• Large Marge (Simpsons episode)
• Large margin nearest neighbor
• Large Margin Nearest Neighbor
• Large margin nearest neighbor classification
• Large Milkweed Bug
• Large Mockolive
• Large mock olive
• Large Mock-olive
• Large Mock Olive
• Large mockolive
• Large Mock olive
• Large Monkey
• Large mouse-eared bat
• Large mouth bass
• Large Mouth Bass
• Largemouth Shiner
• Large Muensterlaender
• Large (music)
• Large Narcissus Fly
• Large Navigational Buoy
• Large ncRNA