“Protein-glutamate methylesterase”的意思、由来-开放百科全书

词条

Protein-glutamate methylesterase

释义

Structural studies
References
Further reading

{{enzyme
| Name = protein-glutamate methylesterase
| EC_number = 3.1.1.61
| CAS_number = 93792-01-9
| IUBMB_EC_number = 3/1/1/61
| GO_code = 0008984
| image =
| width =
| caption =
}}{{Infobox protein family
| Symbol = CheB_methylest
| Name = CheB_methylest
| image = 1a2o structure.png
| width =
| caption = structural basis for methylesterase cheb regulation by a phosphorylation-activated domain
| Pfam = PF01339
| Pfam_clan =
| InterPro = IPR000673
| SMART =
| PROSITE =
| MEROPS =
| SCOP = 1chd
| TCDB =
| OPM family =
| OPM protein =
| CAZy =
| CDD =
}}

In enzymology, a protein-glutamate methylesterase ({{EC number|3.1.1.61}}) is an enzyme that catalyzes the chemical reaction

protein L-glutamate O₅-methyl ester + H₂O protein L-glutamate + methanol

Thus, the two substrates of this enzyme are protein L-glutamate O5-methyl ester and H₂O, whereas its two products are protein L-glutamate and methanol.

This enzyme is a demethylase, and more specifically it belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is protein-L-glutamate-O5-methyl-ester acylhydrolase. Other names in common use include chemotaxis-specific methylesterase, methyl-accepting chemotaxis protein methyl-esterase, CheB methylesterase, methylesterase CheB, protein methyl-esterase, protein carboxyl methylesterase, PME, protein methylesterase, and protein-L-glutamate-5-O-methyl-ester acylhydrolase. This enzyme participates in 3 metabolic pathways: two-component system - general, bacterial chemotaxis - general, and bacterial chemotaxis - organism-specific.

CheB is part of a two-component signal transduction system. These systems enable bacteria to sense, respond, and adapt to a wide range of environments, stressors, and growth conditions.^[1] Two-component systems are composed of a sensor histidine kinase (HK) and its cognate response regulator (RR).^[2] The HK catalyses its own autophosphorylation followed by the transfer of the phosphoryl group to the receiver domain on RR; phosphorylation of the RR usually activates an attached output domain, in this case a methyltransferase domain.

CheB is involved in chemotaxis. CheB methylesterase is responsible for removing the methyl group from the gamma-glutamyl methyl ester residues in the methyl-accepting chemotaxis proteins (MCP). CheB is regulated through phosphorylation by CheA. The N-terminal region of the protein is similar to that of other regulatory components of sensory transduction systems.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes {{PDB link|1A2O}} and {{PDB link|1CHD}}.

References

1. ^{{cite journal | vauthors = Skerker JM, Prasol MS, Perchuk BS, Biondi EG, Laub MT | title = Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis | journal = PLoS Biol. | volume = 3 | issue = 10 | pages = e334 |date=October 2005 | pmid = 16176121 | pmc = 1233412 | doi = 10.1371/journal.pbio.0030334 | url = }}
2. ^{{cite journal | vauthors = Stock AM, Robinson VL, Goudreau PN | title = Two-component signal transduction | journal = Annu. Rev. Biochem. | volume = 69 | issue = | pages = 183–215 | year = 2000 | pmid = 10966457 | doi = 10.1146/annurev.biochem.69.1.183 | url = }}

Structural studies

References

Further reading