“Enterobacteria phage G4”的意思、由来-开放百科全书

词条

Enterobacteria phage G4

释义

Synthetic G4
Phage Therapy
References
External links

{{Taxobox
| name = Enterobacteria phage G4
| virus_group = ii
| familia = Microviridae
| genus = Microvirus
| species = Enterobacteria phage G4
}}Enterobacteria phage G4 is a bacteriophage that infects E. coli.^[1]. First isolated in 1973, the phage was originally isolated from samples of raw sewage and has 5,577 nucleotides. Its isometric capsid contains a single-stranded circular genome of about 5.5 kbp. Phage G4 bears high genetic and structural similarity to the phage ΦX174, though the two phage genomes are up to 39 percent different by sequence.^[1]

Synthetic G4

Synthetic G4 (syn-G4) has the appearance of an icosahedral protein shell and is 50 nm in diameter. has been synthesized through Polymerase Chain Reaction (PCR) assembly of short oligonucleotides. The synthetic phage G4 genomes were effective in E. coli.^[2]

Phage Therapy

Urinary Tract Infections (UTIs) are mainly caused by uropathgenic E. coli, and bacteriophage G4 can be used to treat it. The bacteriophage inject their DNA into the bacteria, releasing many new bacteriophages. This process has many benefits:

The bacteriophage do not attack animal or human cells, only E. coli bacteria.
The bacteriophage reproduce exponentially, so only a small amount is needed.
After the bacteriophage have attached and killed all the bacteria, they disperse throughout the body and are harmless.

References

1. ^{{cite journal|last=Godson|first=G. N.|author2=Barrell, B. G. |author3=Staden, R. |author4=Fiddes, J. C. |title=Nucleotide sequence of bacteriophage G4 DNA|journal=Nature|date=16 November 1978|volume=276|issue=5685|pages=236–247|doi=10.1038/276236a0}}
2. ^¹{{cite journal|last=Yang|first=Ruilin |author2=Han, Yonghua |author3=Ye, Yiwang |author4=Liu, Yuchen |author5=Jiang, Zhimao |author6=Gui, Yaoting |author7=Cai, Zhiming |author8=Wrede, Paul|title=Chemical Synthesis of Bacteriophage G4|journal=PLoS ONE|date=16 November 2011|volume=6|issue=11|pages=e27062|doi=10.1371/journal.pone.0027062}}

External links

[https://www.uniprot.org/taxonomy/10843 UniProt Taxonomy]
[https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=10843 NCBI Taxonomy]
[https://www.ncbi.nlm.nih.gov/nuccore/V00657 NCBI Nucleotide (full genome)]

1 : Microviridae

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