“CLIC5”的意思、由来-开放百科全书

Expression and localization

CLIC5 exists in two alternative splice variants, a smaller CLIC5A and larger CLIC5B protein.

CLIC5A is expressed chiefly in the renal glomerulus, specifically in podocytes. Within the cell, CLIC5A is localized to the plasma membrane and the cytosol, and associates and is regulated by the actin cytoskeleton.^[2] CLIC5A can form ion channels in vitro and its channel activity is regulated by actin, though measurement of its chloride conductance in vitro suggests that CLIC5A is equally selective for cations and anions.

Function

Although chloride intracellular channel (CLIC) proteins were thought to be involved in ion transport in subcellular compartments, their actual functions suggest their role in diverse cellular and physiological functions including apoptosis and angiogenesis in CLIC1.

CLIC5A, through its interactions with the small GTPase Rac1, induces the phosphorylation of ezrin-moeisin-radixin (ERM) proteins and localized production of the phosphoinositide phosphatidylinositol-4,5-bisphosphate.^[3] These two events activate ezrin, enabling it to couple transmembrane proteins to the actin cytoskeleton, which could represent a mechanism by which podocyte foot processes form to enable renal filtration.^[4]

Clinical relevance

CLIC5A deficiency in mouse models potentiates glomerular injury in hypertension. In these mice, podocyte foot processes were also more sparse and disperse than in wild-type mice.^[4]

See also

References

1. ^{{cite journal | vauthors = Berryman M, Bretscher A | title = Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli | journal = Molecular Biology of the Cell | volume = 11 | issue = 5 | pages = 1509–21 | date = May 2000 | pmid = 10793131 | pmc = 14863 | doi = 10.1091/mbc.11.5.1509 }}
2. ^¹{{cite web | title = Entrez Gene: CLIC5 chloride intracellular channel 5| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=53405| accessdate = }}
3. ^{{cite journal | vauthors = Al-Momany A, Li L, Alexander RT, Ballermann BJ | title = Clustered PI(4,5)P₂ accumulation and ezrin phosphorylation in response to CLIC5A | journal = Journal of Cell Science | volume = 127 | issue = Pt 24 | pages = 5164–78 | date = December 2014 | pmid = 25344252 | doi = 10.1242/jcs.147744 }}
4. ^¹{{cite journal | vauthors = Tavasoli M, Li L, Al-Momany A, Zhu LF, Adam BA, Wang Z, Ballermann BJ | title = The chloride intracellular channel 5A stimulates podocyte Rac1, protecting against hypertension-induced glomerular injury | journal = Kidney International | volume = 89 | issue = 4 | pages = 833–47 | date = April 2016 | pmid = 26924049 | doi = 10.1016/j.kint.2016.01.001 }}

Further reading

{{cite journal | vauthors = Singh H | title = Two decades with dimorphic Chloride Intracellular Channels (CLICs) | journal = FEBS Letters | volume = 584 | issue = 10 | pages = 2112–21 | date = May 2010 | pmid = 20226783 | doi = 10.1016/j.febslet.2010.03.013 }}

{{cite journal | vauthors = Maruyama K, Sugano S | title = Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides | journal = Gene | volume = 138 | issue = 1–2 | pages = 171–4 | date = January 1994 | pmid = 8125298 | doi = 10.1016/0378-1119(94)90802-8 }}

{{cite journal | vauthors = Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S | title = Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library | journal = Gene | volume = 200 | issue = 1–2 | pages = 149–56 | date = October 1997 | pmid = 9373149 | doi = 10.1016/S0378-1119(97)00411-3 }}

{{cite journal | vauthors = Shanks RA, Larocca MC, Berryman M, Edwards JC, Urushidani T, Navarre J, Goldenring JR | title = AKAP350 at the Golgi apparatus. II. Association of AKAP350 with a novel chloride intracellular channel (CLIC) family member | journal = The Journal of Biological Chemistry | volume = 277 | issue = 43 | pages = 40973–80 | date = October 2002 | pmid = 12163479 | doi = 10.1074/jbc.M112277200 }}

{{cite journal | vauthors = Suzuki T, Morita R, Sugimoto Y, Sugawara T, Bai DS, Alonso ME, Medina MT, Bailey JN, Rasmussen A, Ramos-Peek J, Cordova S, Rubio-Donnadieu F, Ochoa A, Jara-Prado A, Inazawa J, Delgado-Escueta AV, Yamakawa K | title = Identification and mutational analysis of candidate genes for juvenile myoclonic epilepsy on 6p11-p12: LRRC1, GCLC, KIAA0057 and CLIC5 | journal = Epilepsy Research | volume = 50 | issue = 3 | pages = 265–75 | date = August 2002 | pmid = 12200217 | doi = 10.1016/S0920-1211(02)00052-9 }}

{{cite journal | vauthors = Berryman M, Bruno J, Price J, Edwards JC | title = CLIC-5A functions as a chloride channel in vitro and associates with the cortical actin cytoskeleton in vitro and in vivo | journal = The Journal of Biological Chemistry | volume = 279 | issue = 33 | pages = 34794–801 | date = August 2004 | pmid = 15184393 | doi = 10.1074/jbc.M402835200 }}

{{cite journal | vauthors = Otsuki T, Ota T, Nishikawa T, Hayashi K, Suzuki Y, Yamamoto J, Wakamatsu A, Kimura K, Sakamoto K, Hatano N, Kawai Y, Ishii S, Saito K, Kojima S, Sugiyama T, Ono T, Okano K, Yoshikawa Y, Aotsuka S, Sasaki N, Hattori A, Okumura K, Nagai K, Sugano S, Isogai T | title = Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries | journal = DNA Research | volume = 12 | issue = 2 | pages = 117–26 | year = 2007 | pmid = 16303743 | doi = 10.1093/dnares/12.2.117 }}

{{cite journal | vauthors = Gonzalez A, Ciobanu D, Sayers M, Sirr N, Dalton T, Davies M | title = Gene transcript amplification from cell lysates in continuous-flow microfluidic devices | journal = Biomedical Microdevices | volume = 9 | issue = 5 | pages = 729–36 | date = October 2007 | pmid = 17492382 | doi = 10.1007/s10544-007-9083-1 }}