“Integron”的意思、由来-开放百科全书

Integrons are genetic mechanisms that allow bacteria to adapt and evolve rapidly through the stockpiling and expression of new genes.^[1] These genes are embedded in a specific genetic structure called gene cassette (a term that is lately changing to integron cassette) that generally carries one promoterless ORF together with a recombination site (attC). Integron cassettes are incorporated to the attI site of the integron platform by site-specific recombination reactions mediated by the integrase.

Discovery

Integrons were initially discovered on conjugative plasmids through their role in antibiotic resistance.^[2] Indeed, these mobile integrons, as they are now known, can carry a variety of cassettes containing genes that are almost exclusively related to antibiotic resistance. Further studies have come to the conclusion that integrons are chromosomal elements, and that their mobilisation onto plasmids has been fostered by transposons and selected by the intensive use of antibiotics. The function of the majority of cassettes found in chromosomal integrons remains unknown.

Structure

Gene Cassettes

Additionally, an integron will usually contain one or more gene cassettes that have been incorporated into it. The gene cassettes may encode genes for antibiotic resistance, although most genes in integrons are uncharacterized. An attC sequence (also called 59-be) is a repeat that flanks cassettes and enables cassettes to be integrated at the attI site, excised and undergo horizontal gene transfer.

Occurrence

Integrons may be found as part of mobile genetic elements such as plasmids and transposons. Integrons can also be found in chromosomes.

Terminology

The term super-integron was first applied in 1998 (but without definition) to the integron with a long cassette array on the small chromosome of Vibrio cholerae.^[6]^[7] The term has since been used for integrons of various cassette array lengths or for integrons on bacterial chromosomes (versus, for example, plasmids). Use of "super-integron" is now discouraged since its meaning is unclear.^[6]

References

1. ^{{Cite journal|title=ASMscience {{!}} The Integron: Adaptation On Demand|url=http://www.asmscience.org/content/journal/microbiolspec/10.1128/microbiolspec.MDNA3-0019-2014|doi=10.1128/microbiolspec.mdna3-0019-2014}}
2. ^{{cite journal | last1 = Mazel | first1 = | year = 2006 | title = Integrons: agents of bacterial evolution | url = | journal = Nature Reviews Microbiology | volume = 4 | issue = 8| pages = 608–620 | doi = 10.1038/nrmicro1462 }}
3. ^{{cite journal|doi=10.1023/A:1015361704475|year=2002|last1=Kovalevskaya|first1=N. P.|journal=Molecular Biology|volume=36|issue=2|pages=196–201|title=Mobile Gene Cassettes and Integrons}}
4. ^Hall R, Collis C, Kim M, Partridge S, Recchia G, Stokes H (1999) Mobile gene cassettes and integrons in evolution.
5. ^{{cite journal|pmid=7783631|year=1995|last1=Hall|first1=RM|last2=Collis|first2=CM|title=Mobile gene cassettes and integrons: Capture and spread of genes by site-specific recombination|volume=15|issue=4|pages=593–600|journal=Molecular Microbiology|doi=10.1111/j.1365-2958.1995.tb02368.x }}
6. ^¹{{cite journal|doi=10.1099/mic.0.26854-0|title=Integrons or super integrons?|year=2004|last1=Hall|first1=R. M.|journal=Microbiology|volume=150|pages=3–4|pmid=14702391|last2=Stokes|first2=HW|issue=Pt 1}}
7. ^{{cite journal|doi=10.1126/science.280.5363.605|title=A Distinctive Class of Integron in the Vibrio cholerae Genome|year=1998|last1=Mazel|first1=D.|journal=Science|volume=280|issue=5363|pages=605–8|pmid=9554855|last2=Dychinco|first2=B|last3=Webb|first3=VA|last4=Davies|first4=J}}