Ambartsumian (or Ambartsumyan) is an Armenian surname. Ambartsumian may refer to:

• Armen Ambartsumyan (born 1978), Bulgarian-Armenian footballer
• David Ambartsumyan (1956–1992), Armenian diver
• Grigori Ambartsumyan (1933–2014), Soviet footballer and coach
• Levon Ambartsumian (born 1955), Armenian violinist and conductor
• Victor Ambartsumian (1908–1996), Armenian-Russian astronomer and astrophysicist
• Vyacheslav Ambartsumyan (1940–2008), Soviet international footballer

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