词条 | Super Optimal Broth |
释义 |
Composition(Figures in parentheses are the masses required to create 1 liter of medium) SOB
SOCIn addition to the SOB contents, SOC also contains 20mM glucose (3.603 g) Alternatively, SOB and SOC can be made by adding small amounts of concentrated magnesium chloride and glucose solutions to pre-prepared SOB. pH adjustmentFor maximum effectiveness, SOB/SOC media should have its pH adjusted to 7.0 by adding concentrated sodium hydroxide.[3] The original literature states that the pH of the final medium should be between 6.8 and 7.0. SterilizationFinally, the SOB medium should be autoclaved at 121 °C to ensure sterility. The components of SOC medium should not be autoclaved together because the high temperature can cause the glucose to react with tryptic peptides (see Maillard reaction), compromising the quality. SOB and magnesium and glucose additive solutions can be autoclaved separately and mixed afterwards to final concentrations. Complete SOC can be filter sterilized through a 0.22 µm filter. References1. ^{{Cite journal | last1 = Hanahan | first1 = D. | title = Studies on transformation of Escherichia coli with plasmids | journal = Journal of Molecular Biology | volume = 166 | issue = 4 | pages = 557–580 | year = 1983 | pmid = 6345791 | doi=10.1016/S0022-2836(83)80284-8}} 2. ^1 SOC Medium 3. ^1 4. ^SOC medium 2 : Microbiological media|American inventions |
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